Alltech 3300 ELSD
The Model 3300 ELSD ( is designed for use with High Performance Liquid Chromatography(HPLC) systems to analyze any sample compound that has sufficiently lower volatility than the mobile phase.
Some of its possible application areas include carbohydrates, pharmaceuticals, lipids, triglycerides, underivitized fatty and amino acids, polymers, surfactants, nutraceuticals, and combinatorial libraries.
Evaporative light scattering detection eliminates common problems associated with other HPLC detectors. Refractive Index (RI) detectors can be complicated by solvent front interferences, baseline instability, and gradient incompatibility. RI detectors can also have a less sensitive response than ELSD. Low-wavelength UV can suffer baseline drift with steep gradients and also requires that the analyte contains a chromophore. ELSD does not have these limitations. It can achieve stable baselines with multisolvent gradients for improved resolution and faster separations. Also, since ELSD response does not depend on the sample’s optical characteristics, the sample does not require a chromophore or fluorophore for detection.
The Model 3300 ELSD features the most advanced evaporative light scattering detection technology available. It provides sensitivity in the low nanogram range. An intuitive, Windows -based software interface provides a nested menu of options for detector operation, including Method Wizard for simplified method development, multiple languages, and builtin Diagnostic tests for troubleshooting. The Model 3300 ELSD also features compact, stackable dimensions, allowing it to fit easily into limited laboratory space.
PRINCIPLE OF OPERATION
The unique detection principle of evaporative light scattering detection involves a three-step process: nebulization of the column effluent to form an aerosol, solvent evaporation within a heated drift tube, and detection of the non-volatile solute particles in the light scattering cell.
*(2020, March 3) Alltech_Model_3300_ELSD_Operation_Manual_en.pdf