Description

Objective Agilent 6490 

To show the high sensitivity of the 6490 Triple Quadrupole LC/MS System with iFunnel technology for quantitation of peptide samples in plasma.

Background Agilent 6490

The Agilent 6490 Triple Quadrupole mass spectrometer incorporates iFunnel technology to achieve new levels of sensitivity and dynamic range for detection of target peptides in complex matrices such as human plasma. The innovative design of the ion funnel reduces contamination and neutral molecules thus improving overall signal and reducing system noise.

Sensitivity is the key requirement for assays that are both specific and quantitative for target proteins. These assays are critical for clinical research of putative biomarkers, which may have low concentrations in commonly used bioflu ids such as serum and plasma. Often hundreds of target peptides need to be monitored in thousands of biological samples.

This note describes the results achieved for the analysis of stable isotope-labeled standard peptides spiked into digested non-depleted plasma using the 6490 Triple Quadrupole LC/MS System with iFunnel technology.

Key Benefits Agilent 6490 

• The results show attomole level sensitivity for quantitation of peptide samples in plasma using standard flow LC
• 110 replicate injections of peptides in plasma demonstrated robustness and reproducibility for these sample types
• The 6490 with iFunnel technology is ideal for the detection of target peptides in complex matrices such as human plasma

source: © Agilent Technologies, Inc. 2010 Published in the U.S.A. September 15, 2010 5990-6513EN