Description

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Agilent G1362A 1260 Refractive Index Detector (RID)

The Agilent G1362A 1260 RID is a Refractive Index Detector designed for use in High-Performance Liquid Chromatography (HPLC) systems. It is particularly useful for analyzing compounds that do not exhibit UV absorbance, making it a universal detector suitable for a broad range of analytes. Typical applications include the detection of polymers, carbohydrates, triglycerides, and organic acids.

This detector operates by measuring changes in the refractive index of the mobile phase as solutes elute from the column, providing a sensitive and stable signal for non-UV-active compounds. Due to its high sensitivity and stability, the 1260 RID is especially well-suited for Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC), where accurate molecular weight distribution analysis is required.

Key Features and Functions of the Agilent G1362A 1260 Refractive Index Detector (RID)

Detection Method:
The detector operates by measuring the difference in refractive index between the mobile phase and the eluting sample as they pass through a precision flow cell. This universal detection principle enables analysis of compounds that do not absorb UV or visible light.

Applications:
The 1260 RID is ideal for detecting non-UV-active compounds such as polymers, carbohydrates, sugars, organic acids, and lipids. It is widely used in Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) for molecular weight and polymer distribution analysis.

Stability and Performance:
The system incorporates optics temperature control and automatic light intensity adjustment, ensuring high baseline stability and reproducible results even under changing ambient conditions.

Automated Functions:
To simplify operation and improve efficiency, the detector includes several automated features:

• Automatic zeroing before each analysis
• Programmable reference cell purge for maintaining performance over time
• Optional solvent recycle function, reducing solvent consumption and operating costs

Compatibility:
The Agilent G1362A RID is fully integrated within the Agilent 1260 Infinity series of HPLC systems. It supports both entry-level UHPLC and conventional HPLC applications, providing flexibility for a wide range of analytical workflows.