Recently purchased new, an Agilent 1200 system with a 1260 Infinity fraction collector.
Agilent HPLC 1200 series + 1260 Infinity G1364A 1260 FC-AS
Comprises a 4-channel vacuum container, including 4 micro structured membranes, and a vacuum pump. When the micro vacuum degasser is switched on, the control circuit turns on the vacuum pump, which is operated with constant speed and voltage generates a partial vacuum in the vacuum container. The pressure is measured by a pressure sensor. The micro vacuum degasser maintains the partial vacuum with the help of a controlled leak in the degasser’s proportional valve and by varying size of the controlled leak within the proportional valve, depending on the signal from the pressure sensor.
The LC pump draws the solvents from their bottles through the special plastic membranes of the vacuum container. As the solvents pass through the vacuum chambers any dissolved gas in the solvents permeates through the membranes into the vacuum container. The solvents will be almost completely degassed when leaving the outlets of the micro vacuum degasser.
This Binary Pump features a two-channel, dual-piston in-series design which comprises all essential functions of a solvent delivery system. Metering of solvent and delivery to the high-pressure side are performed by two pump assemblies which can generate pressure up to 400 bar. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve, and outlet valve. Both channels are connected in a low-volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer.
The design of the Agilent 1200 G1312B SL with variable stroke volume allows pulse-free solvent delivery and efficient mixing. An inlet-valve electronically activated and synchronized to piston stroke-eliminates vapor formation with volatile solvents and is maintenance free.
The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation for LC systems. The specially-designed thermostattable sample trays holds either 100 × 1.8 ml vials or two wellplates and 10 × 1.8 ml vials. The autosampler thermostat contains Peltier-controlled heat exchangers. A fan draws air from the area above the sample vial tray of the autosampler and is then blown through the fins of the cooling/heating module. There it is cooled or heated according to the temperature setting. The thermostatted air enters the autosampler through a recess underneath the specially-designed sample tray. The air is then distributed evenly through the sample tray ensuring effective temperature control, regardless of how many vials are in the tray. In cooling mode condensation is generated on the cooled side of the Peltier elements. This condensed water is safely guided into the leak system.
Controls temperature for either HPLC’s or fraction collectors. The ALS thermostat contains Peltier-controlled heat exchangers. A fan draws air from the area above the sample vial tray of the autosampler and is then blown through the fins of the cooling/heating module. There it is cooled or heated according to the temperature setting. The thermostatted air enters the autosampler through a recess underneath the specially-designed sample tray. The air is then distributed evenly through the sample tray ensuring effective temperature control, regardless of how many vials are in the tray.
The Analytical-Scale Fraction Collector is optimized for laboratories performing analytical-scale compound purification of nanogram to milligram quantities with typical flow rates between 0.1 and 10 mL/min. The module features the full set of collection modes and is compatible with a wide variety of trigger sources. SBS plates and four different tube sizes with various lenghts are supported.
The Analytical-Scale Fraction Collector features a compact closed-box design, requires minimal bench space, and can be seamlessly integrated into any InfinityLab Analytical System. The built-in fraction delay sensor allows automated delay calibration for maximum substance recovery, and the four preconfigured capillary kits ensure minimum peak dispersion for maximum purity. Maximum capacity can be easily enhanced by clustering up to three modules.
This verifies separation quality with peak purity, multiple signals, and spectral libraries during analytical quantification, offering an extended wavelength range with the highest sensitivity. The diode array detector allows for automated wavelength verification for performance tracking.
Ultra fast separations can generate sharp and narrow peaks with peak widths smaller than 1 second. The Agilent 1200 Series DAD SL offers multiple wavelength and full spectral detection at up to 80 Hz sampling rate, keeping up with the analysis speed of ultra-fast LC. Even when operated at high data rates, the 1200 Series DAD SL allows precise identification, quantification and peak purity analysis at trace levels. This exceptional capability assures that your ultra-fast LC methods comply with the strictest regulatory performance requirements.
The Agilent 1200’s temperature management system ensures optimum baseline stability, even under rough ambient conditions. This detector features superb illumination using combined deuterium and tungsten lamps for the highest intensity and lowest detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest spectral resolution. The programmable slit helps you explore the spectral landscape for faster sample characterization, ensuring higher sensitivity or lower baseline noise as necessary on the 1100. Slit settings can be stored and reported from 1 – 16 nm with raw data for GLP traceability.